streptavidin donor beads (Revvity)
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Revvity
streptavidin donor beads
Streptavidin Donor Beads, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+donor+beads/Aluminum/us11827642-846-5-8
Average 91 stars, based on 151 article reviews
Streptavidin Donor Beads, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+donor+beads/Aluminum/us11827642-846-5-8
Average 91 stars, based on 151 article reviews
streptavidin donor beads - by Bioz Stars,
2026-10
91/100 stars
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Incubation:Article Title: HIV-1 neutralizing antibodies elicited in humans by a prefusion-stabilized envelope trimer form a reproducible class targeting fusion peptide Article Snippet: To each well was added 10 μL of biotinylated Env trimer probe at 10 nM final concentration and 10 μL of anti-human Fc IgG (PerkinElmer, Waltham, MA) acceptor beads at 10 μg/mL final concentration. .. The mixtures were incubated at room temperature (RT) for an hour and then added 25 μL of Article Title: A Cryptic Basic Groove formed by Ubiquitin and Histone H3 Mediates the Selective Recognition of H2AK119Ub Nucleosomes by Synovial Sarcoma X Breakpoint 1 Protein Article Snippet: .. 10 μL of 200 nM (or serially diluted) GST-tagged protein (or its mutants) and 10 μL of 12 nM biotinylated nucleosome (or its mutants) were mixed and incubated in OptiPlate-384 microplate (PerkinElmer) for 60 mins, then 10 μL of 20 μg/mL glutathione acceptor beads (PerkinElmer, AL109C, Lot: 2851471) and 10 μL 20 μg/mL Article Title: AI-based antibody discovery platform identifies novel, diverse and pharmacologically active therapeutic antibodies against multiple SARS-CoV-2 strains Article Snippet: Subsequently, 10 μl of Protein A AlphaLISA ® Acceptor beads (10 μg/ml final concentration; Perkin Elmer cat# AL101C) were added, and further incubated for 1 hour at room temperature in the dark with shaking (250 rpm). .. Following this incubation, 20 μl of Article Title: The Parallel Structure–Activity Relationship Screening of Three Compounds Identifies the Common Agonist Pharmacophore of Pyrrolidine Bis-Cyclic Guanidine Melanocortin-3 Receptor (MC3R) Small-Molecule Ligands Article Snippet: Briefly, cells 70−90% confluent were dislodged with Versene (Gibco, Waltham, MA, USA) at 37 °C and 10,000 cells/well were plated in a 384-well plate (Optiplate, PerkinElmer, Waltham, MA, USA) with 10 μL of freshly prepared stimulation buffer (1× HBSS, 5 mM HEPES, 0.5 mM IBMX, 0.1% BSA, pH = 7.4) with 0.5 μg of anti-cAMP acceptor beads per well. .. The cells were stimulated with the addition of 5 μL of stimulation buffer containing compound or forskolin (10 −4 M) and incubated in the dark at room temperature for 2 h. Following stimulation, Concentration Assay:Article Title: HIV-1 neutralizing antibodies elicited in humans by a prefusion-stabilized envelope trimer form a reproducible class targeting fusion peptide Article Snippet: To each well was added 10 μL of biotinylated Env trimer probe at 10 nM final concentration and 10 μL of anti-human Fc IgG (PerkinElmer, Waltham, MA) acceptor beads at 10 μg/mL final concentration. .. The mixtures were incubated at room temperature (RT) for an hour and then added 25 μL of Article Title: AI-based antibody discovery platform identifies novel, diverse and pharmacologically active therapeutic antibodies against multiple SARS-CoV-2 strains Article Snippet: Subsequently, 10 μl of Protein A AlphaLISA ® Acceptor beads (10 μg/ml final concentration; Perkin Elmer cat# AL101C) were added, and further incubated for 1 hour at room temperature in the dark with shaking (250 rpm). .. Following this incubation, 20 μl of Amplified Luminescent Proximity Homogenous Assay:Article Title: Small molecule branched-chain ketoacid dehydrogenase kinase (BDK) inhibitors with opposing effects on BDK protein levels Article Snippet: .. Compounds were pre-incubated with BDK prior to the addition of the E2 subunit and signal was developed with the addition of 1:1000 CaptSure tagged Ms Mono anti-DBT antibody (Novus), Anti CaptSure acceptor beads and Article Title: Assay for determining antibody or ligand binding and function Article Snippet: .. For the AlphaScreen based method, biotinylated WGA was bound to Adhesive:Article Title: Compositions useful for treating disorders related to KIT Article Snippet: After 90 minutes, 6 ul of 5X AlphaLISA Lysis Buffer (Perkin Elmer) supplemented with a protease and phosphatase inhibitor cocktail (Cell Signaling Technologies) was added to each well and shaken at 450 rpm for 15 minutes at 4° C. 10 ul of phospho-Y719 c-KIT and total c-KIT antibodies (15 nM final concentration, Cell Signaling Technologies) and 50 ug/ml AlphaLISA rabbit acceptor beads (Perkin Elmer) were added to each well and shaken at 300 rpm at room temperature for 2 hours. .. 10 ul of 100 ug/ml Whole Genome Amplification:Article Title: Assay for determining antibody or ligand binding and function Article Snippet: .. For the AlphaScreen based method, biotinylated WGA was bound to Binding Assay:Article Title: Assay for determining antibody or ligand binding and function Article Snippet: .. For the AlphaScreen based method, biotinylated WGA was bound to Lysis:Article Title: The Parallel Structure–Activity Relationship Screening of Three Compounds Identifies the Common Agonist Pharmacophore of Pyrrolidine Bis-Cyclic Guanidine Melanocortin-3 Receptor (MC3R) Small-Molecule Ligands Article Snippet: Briefly, cells 70−90% confluent were dislodged with Versene (Gibco, Waltham, MA, USA) at 37 °C and 10,000 cells/well were plated in a 384-well plate (Optiplate, PerkinElmer, Waltham, MA, USA) with 10 μL of freshly prepared stimulation buffer (1× HBSS, 5 mM HEPES, 0.5 mM IBMX, 0.1% BSA, pH = 7.4) with 0.5 μg of anti-cAMP acceptor beads per well. .. The cells were stimulated with the addition of 5 μL of stimulation buffer containing compound or forskolin (10 −4 M) and incubated in the dark at room temperature for 2 h. Following stimulation, |